Rubisco activase chaperone activity is regulated by a post-translational mechanism in maize leaves

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Rubisco activase chaperone activity is regulated by a post-translational mechanism in maize leaves.

Rubisco activase (RCA) is a molecular chaperone present in maize as 43 kDa and 41 kDa polypeptides. They are encoded by two different genes comprising an identical ORF that corresponds to the 43 kDa RCA polypeptide, and their transcripts do not show putative splicing sites. To determine the origin of the 41 kDa polypeptide, leaf poly A(+) mRNA was in vitro translated. Results demonstrated de no...

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Purification and assay of rubisco activase from leaves.

Ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) activase protein was purified from spinach leaves by ammonium sulfate precipitation and ion exchange fast protein liquid chromatography. This resulted in 48-fold purification with 70% recovery of activity and yielded up to 18 milligrams of rubisco activase protein from 100 grams of leaves. Based on these figures, the protein comprised ap...

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Rubisco, Rubisco activase, and global climate change.

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ژورنال

عنوان ژورنال: Journal of Experimental Botany

سال: 2004

ISSN: 1460-2431

DOI: 10.1093/jxb/erh268